Reliable Method for Obtaining Serial Ultrathin Sections Using New Small Tools
Abstract: Procedure of obtaining serial ultrathin sections for electron microscopy consists of several steps: cutting of uniform very thin sections by ultramicrotome; making ribbon sections for taking pictures in electron microscope one after another; separating ribbon sections to fixed length for mounting grids; and collecting ribbons on the grids with no failure. The present report describes a reliable method for obtaining serial ultrathin sections from a resin block of biological tissues using new small tools.
Key words: block face preparation, trimming method, separation of ribbon sections, section collection method, small tools