KENBIKYO Vol.49▶No.3 2014
â– Lectures

Electron Microscopic Analysis of Rice Seed

Takehiro Masumura, Ai Sasou and Yuhi Saito

Abstract: Morphological observation of organelle in the mature rice seed is poorly understanding, because the electron microscopy of mature rice seed is technically difficult. We attempted to modify the existing method of embedding rice grain in resin. Modified method revealed the ultrastructures of mature organelle in dry rice seed. Observation of subcelluler structures in mature starchy endosperm in rice grains will be useful in future to farmers, breeder, consumers and food processors. Moreover, this method should provide a highly useful tool for observation of organelle and understanding seed development of other cereals.

Key words: Rice seed, TEM, storage protein, protein body