KENBIKYO Vol.48▶No.1 2013
â– Researches Today

Application of Direct Electron Detector for TEM

Naoyuki Miyazaki and Kazuyoshi Murata

National Institute for Physiological Sciences

Abstract: Direct electron detector (DED) cameras using CMOS image sensor are developed and used as an imaging media in transmission electron microscope (TEM). Since images recorded in DED show higher spatial resolution and better signal to noise ratio than those in CCD, it is applied to low dose imaging, especially for cryo-electron microscopy. The fast readout speed of DED is also used to a time-lapse recording and a specimen drift correction. In this article, we describe the principle and the performance of DED, and introduce the recent applications.

Key words: CMOS image sensor, CryoEM, High-seed recording, CCD, Specimen drift correction