MICROSCOPY Vol.44▶No.2 2009
â– Lectures

Three-dimensional Imaging Characteristics in Imaging Optical Systems

Takashi Ikuta

Abstract: In this lecture, a concept of three-dimensional imaging characteristics of optical imaging systems, applicable to both optical and electron microscopes, are described with a demonstration of image observations using a transmission optical microscope. These experimental results are, then, interpreted and discussed on the basis of the three-dimensional optical transfer function (3D-OTF) of the transmission microscopes. Finally, new imaging techniques, using active control for the illumination/imaging systems followed by successive image processing, are proposed to improve the tree-dimensional imaging characteristics of the original microscope.

Key words: Electron microscope, Optical Microscope, Three-dimensional imaging theory, Three-dimensional imaging characteristics, Optical transfer function