Development of Low Energy Holography Electron Microscope

Tsumoru Shintake, Hidehito Adaniya, Masao Yamashita and Martin Phillip Cheung

Abstract: In order to obtain higher contrast image from bio-nano particles, we have developed electron microscope with lower beam energy. Additionally, we employed employing in-line holography. By means of FFT based image reconstruction, we may obtain complex object amplitude, thus phase object image at higher contrast. We started to observe bio-nano particle at nano-meter resolution.We are currently focus to improve sample preparation technique.

Key words: In-line holography, Gabor holography, low energy electron microscope, electron diffraction