KENBIKYO Vol.52▶No.2 2017

Immunogold Labeling for Plant Tissues and Cells

Mayuko Sato, Yumi Goto and Kiminori Toyooka

Abstract: Immunogold labeling for electron microscopy is a powerful technique to identify the localization of molecules in tissues and cells. Sample preparation of plants requires careful methods because plant cells have specific organelles that are difficult to fix and to infiltrate resin into, such as thick cell walls, large vacuoles and hard starch granules. In this paper, we introduce the techniques and methods for immunogold labeling of plant organs, tissues and cells. Moreover, we give some examples of immunogold labeling using plant samples.

Key words: Plant, Immunoelectron microscopy, Imunnogold labeling, High-pressure freezing/freeze substitution