KENBIKYO Vol.53▶No.1 2018

Microscopic Study in Bone Biology and Medicine

Tadahiro Iimura and Ji-Won Lee

Abstract: Bone is one of the hard tissues in living vertebrates. Homeostasis of the skeletal system, bone morphology and mineral metabolism, which is called bone metabolism, is highly regulated through balancing between bone resorption and bone formation. Bone metabolism is tightly associated with histological morphology of the bone. Therefore, microscopic analysis has been a gold-standard approach for understanding bone pathophysiology. This short technical review scopes the application of current microscopic innovations including super-resolution microscopy (especially, structured illumination microscope) to the bone biomedicine by introducing our recent works.

Key words: bone metabolism, osteoclasts, differential interference contrast microscope, deconvolution, structured illumination microscope